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الكشف عن جينات مقاومة مغزلاويه الذبول المرض في أصناف الحمص وبنود استخدام الواسمات الجزيئية- (Arabic)

Somayeh Farahani, Bahar Morid, Mojdeh Maleki

Department of Plant Protection, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

Department of Plant Protection, College of Agriculture, Takestan Branch, Islamic Azad University, Takestan, Iran

Key words: Chickpea, Fusarium wilt, Molecular markers, Race zero, RAPD.

Abstract

Fusarium wilt is reported to infect crops in over 32 countries all around the world; reducing 10-90% of annual crop yield. The ability of the pathogen to survive in soil for several years, even without the host, makes it really difficult to control. However, using chickpea cultivars resistant to Fusarium wilt is the most effective and environmentally friendly method of preventing yield loss. Phenotypic evaluation of germplasm and improved lines to find resistance to specific race of the pathogen is a time consuming and expensive method which is affected by the amount of inoculum and environmental conditions. In this experiment, molecular marker RAPD was used to detect chickpea genotypes containing the resistance gene. The DNA of 42 chickpea genotypes was extracted by CTAB method. Then, polymerase chain reaction was conducted using OPG-20 marker. At the end, results were confirmed by the pathogenesis test. Results indicated that 40 genotypes out of the 42 were sensitive to race zero and only KC216194 mass and Jam cultivar were resistant to race zero. The pathogenesis test confirmed the results of genetic evaluations.

Sources: http://www.innspub.net/wp-content/uploads/2015/01/IJB-V6No1-p29-35.pdf

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