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Antioxidant effect of Peganum harmala L on toxicity caused by silver nanoparticles in mice liver

Samira Karamsichani, Ali Noori, Nooshin Naghsh, Mohammad Reza Aghaye Ghazvini

Department of Biochemistry, Science and Research Branch, Islamic Azad University, Fars, Iran

Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Isfahan Center of Health Research, National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran

Key words: Peganum harmala L (Ph), Nanosilver particles (Ns), Catalase, Glutathione peroxidase, Malondialdehyde, Liver tissue.


Peganum_-mexicanum-790The research objective is to examine the effect of Ph (Peganum harmala L) ethanolic extract on liver tissue, malondialdehyde density, catalase and glutathione peroxidase activity of male mice, treated by nanosilver particles. The research was conducted on adult male mice of albino race (25-30gr). The animals were divided into four groups. Group 1, as control, Group 2 were treated by nanosilver particles, Group 3 received 20mg/kg/day of oral Ph ethanolic extract for 30 days. Group 4 is the composition of the Group 2 and 3. The activity rate of catalase (CAT) serum and glutathione peroxidase (Gpx) of red blood cells, density of malondialdehyde (MDA) serum were determined .The collected sections of liver tissue were stained with hematoxylin and eosine in order to be studied. The liver tissue of mice treated with nanosilver particles and Ph ethanolic extract, was normal as it was in control group. Malondialdehyde showed significant decrease, and catalase and glutathione peroxidase activity increased significantly (p<0/001) in comparison with group 2 (treated with nanosilver particles 500NS). The results of this research show that antioxidant property of Ph ethanolic extract prevents liver damage caused by free radicals resulting from silver nanoparticles. It can even be introduced as a reducer of free radicals induced by silver nanoparticle.


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