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Comparison of Ascochyta rabiei isolates for cultural characteristics and isozyme

Gharbi Samia, Karkachi Noureddine, Chhiba Mostafa, Kihal Merbrouk, Henni Jamal Eddine.

Department of Biotechnology, University of Sciences and the Technology of Oran, Algeria

Department of Biology, University of Oran, Algeria

Faculty of Sciences and Technology, University HASSAN 1st, Settat, Morocco

Key words: Ascochyta raiei, Esterase, Acid phosphatase, Superoxide dismutase, Leucine aminopeptidase.


chickpea6The comparison of our isolates on the basis of macroscopic and microscopic criteria reveals differences between 32 isolates of Ascochyta rabiei, it is distributed as follows, light brown, dark brown and green (50, 22 and 28%). Pathogenicity is performed on three varieties of chickpea (Flip 90-13, Flip 33-99 and ILC 1799), which showed no aggressiveness with the two varieties Flip 90-13, but with the variety ILC 1799 showed a pathological variability of 10 isolates of Ascochyta rabiei. Electrophoretic study of five isoenzymes esterase (Est), acid phosphatase (CAP), leucine aminopeptidase (LAP), superoxide dismutase (SOD), peroxidase (POX) showed that only Esterase and acid phosphatase isoenzyme have an polymorohisme. A dendrogram is established, integrating all the data. It reveals the importance of genome diversification within the species.

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