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Designing streptococcus iniae diagnostic kit by multiplex polymerase chain reaction method in Iranian rainbow trout

F.Yadollahi, A. Haghighi Khiabanian Asl, B. Kazemi, M. Afsharnasab

Department of Aquatic Animal Health and Disease, Science and Research Branch, Islamic Azad University, Tehran, Iran

Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, IrCellular and Molecular Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Key words: Streptococcosis, Rainbow Trout, Multiplex PCR, Control.

Abstract

steelhead-Rainbow trout jumping_2Streptococcus is a large genus of bacteria which cause Streptococcosis in aquatic animals. In Iranian fishes, Streptococcus iniae cause Streptococcosis. This disease was formerly diagnosed as golf ball disease. The current study aimed to produce a polymerase chain reaction (PCR) kit with five local suspicious internal controls with clinical symptoms such as melanosis, pallor, exophthalmia and bleeding in the anterior part of the eye and retina, fin base, vent, and gills through histopathologic technique to diagnose Streptococcosis using MultiPlex PCR method. In this study, two bands of 725 base pair and 340 bp indicating the host (control) and the pathogen bacteria were observed, the PCR was confirmed and the Streptococcosis in the suspicious samples was diagnosed. The aim of this study demonestrated designig new kit for diagnosed the diagnose Streptococcosis using to MultiPlex PCR method.

Get the original articles: http://www.innspub.net/volume-6-number-3-february-2015-ijb/

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