Hamid Nouri, Saeid Navabpour, Ahad Yamchi, Farhood Zeyaee
Department of Plant Breeding and Biotechnology, Gorgon university of Agricultural Sciences and Natural Resources, Gorgan, Iran
Agricultural, Medical and Industrial Research, Nuclear Sciences and Technology Research Institute, Karaj, Iran
Key words: Antioxidant Enzymes, Salinity, Mutant Line, Lipid Peroxidation, Wheat.
Effect of sodium chloride salinity was studied in advanced mutant lines T-65-7-1 and T-67-60 (tolerant) and their wild type Tabasi (moderately tolerant) wheat genotypes under control and level of salinity (EC 6 ds m-1) at seedling stage. Salinity treatment decreased the contents of chlorophyll (CHL), carotenoids (CAR), α-Tocopherol (TOC) as well as enzyme activities including catalase (CAT), superoxide dismutase (SOD), ascorbic acid (AA), ascorbate peroxidase (APOX), and glutathione reductase (GR), whereas increased the contents of superoxide ion (O2 -), hydrogen peroxide (H2O2), thiobarbituric acid reactive material (TBARM) (measuring of lipid peroxidation) in wild type Tabasi cultivar. Salinity tolerant mutant line T-67-60 showed more enzymatic activities (CAT, SOD, ASA, APOX, GR) as well as CHL, CAR and TOC contents than T-65-7-1 mutant line and Tabasi cultivar. Interestingly, the contents of O2 -, H2O2 and TBARM in T-67-60 line showed less increased than T-65-7-1 line and Tabasi cultivar by salinity treatment. Base on these results the tolerance of T-67-60 mutant line to salinity, justified by intensified enzyme activities to control oxidative damage via quench ROS levels.
Get the original articles: http://www.innspub.net/volume-6-number-4-february-2015-ijb/