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Effect of saltiness on the growth and production of β-carotene in isolated Dunaliella sp. microalga from qom salt lake of Iran

Faraz Arash Rad, Nilufer Aksoz, Mohammad Amin Hejazi

Department of Food Science, Mahabad Branch, Islamic Azad University, Mahabad, Iran

Department of Biology- Biotechnology, Faculty of Science, Hacettepe University, Beytepe 06800, Ankara, Turkey

Agricultural Biotechnology Research Institute, Northwest Branch, Tabriz, Iran

Key words: Dunaliella, Qom Salt Lake, Microalgae, β-carotene.

Abstract

5660752The aim of this study was to achieve the highest β-carotene production by Dunaliella sp. isolates from under salinity stress factor. In this study, water samples from hyper-saline Qom Salt Lake were cultured in modified Johnson media and were treated at different salinities (1, 2 and 3 M of NaCl). In order to determine the optimal salinity required for the highest β-carotene accumulation, cell count of Dunaliella sp. isolates; total carotenoids and concentration of the β-carotene were determined by direct microscopic counting and spectrophotometry. In the samples with different salinities, the cell count and the β-carotene content of Dunaliella sp. ranged between 0.46 and 2.12×106 cell.ml-1 and 0.16 to 9.88 pg.cell-1, respectively. At the end of the experiments, the maximum cell content mean and the highest β-carotene content mean were obtained at 2 and 3 M NaCl concentrations, as 1.78×106 cell.ml-1 and 7.40 pg.cell-1, respectively.

Source: http://www.innspub.net/wp-content/uploads/2015/01/IJB-V6No2-p164-171.pdf

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