Faraz Arash Rad, Nilufer Aksoz, Mohammad Amin Hejazi
Department of Food Science, Mahabad Branch, Islamic Azad University, Mahabad, Iran
Department of Biology- Biotechnology, Faculty of Science, Hacettepe University, Beytepe 06800, Ankara, Turkey
Agricultural Biotechnology Research Institute, Northwest Branch, Tabriz, Iran
Key words: Dunaliella, Qom Salt Lake, Microalgae, β-carotene.
The aim of this study was to achieve the highest β-carotene production by Dunaliella sp. isolates from under salinity stress factor. In this study, water samples from hyper-saline Qom Salt Lake were cultured in modified Johnson media and were treated at different salinities (1, 2 and 3 M of NaCl). In order to determine the optimal salinity required for the highest β-carotene accumulation, cell count of Dunaliella sp. isolates; total carotenoids and concentration of the β-carotene were determined by direct microscopic counting and spectrophotometry. In the samples with different salinities, the cell count and the β-carotene content of Dunaliella sp. ranged between 0.46 and 2.12×106 cell.ml-1 and 0.16 to 9.88 pg.cell-1, respectively. At the end of the experiments, the maximum cell content mean and the highest β-carotene content mean were obtained at 2 and 3 M NaCl concentrations, as 1.78×106 cell.ml-1 and 7.40 pg.cell-1, respectively.