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Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji – JBES

Rhimi Awatef, Boussaid Mohamed

Laboratory of Plant Biotechnology, National Gene Bank of Tunisia (NGBT). Boulevard of the Leader Yasser Arafat, ZI Charguia 1, 1080 Tunis Cedex, Tunisia

Department of Biology, Laboratory of Plant Biotechnology. National Institute of Applied Sciences and Technologies (INSAT). Centre Urbain Nord, B.P. 676, 1080 Tunis Cedex, Tunisia

Key words: Buds, caulogenesis, Cucumis melo L. cv. Maazoun and Beji, plant regeneration, tissue culture.


A procedure for the regeneration of melon (Cucumis melo L.) cv. Maazoun and Beji via shoot organogenesis from cotyledon explants is described. Cotyledons explants cultivated on the basal Murashige and Skoog (1962) medium, added with combinations of BAP, NAA and 2,4-D developed adventitious shoots after two weeks. The rates of caulogenesis varied according to the medium hormonal composition and the genotype. The best induction medium for a morphogenic response was MS containing 0.5 mgl-1 NAA and 2 mgl-1 BAP. The highest caulogenesis percentages were 79.16% and 58.33% for Maazoun and Beji cultivars respectively. The mean number of buds per callus was 2.92. Development phases were characterized by histological studies. Some phases of shoot organogenesis were observed in Cucumis melo L. Maazoun and Beji cultivars. The shoots were rooted in MS medium without growth regulators or added NAA (0.5 or 1 mgl-1). The highest bud rooting of buds occurred on MS medium added with 1 mg/l NAA. The percentage of rooting varied from 16.66 to 75%. The highest percentage of survival plants after acclimatization were 41.73 and 57.63 for Beji and Maazoun respectively.

Get the original articles in Source: Archive for | JBES |- February, 2016

Journal Name: Journal of Biodiversity and Environmental Sciences (JBES)

Published By: International Network for Natural Sciences

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