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Evaluation of microbiological quality of bottled water to Pseudomonas aeruginosa by membrane filter, culture and polymerase chain reaction method

Fatemeh Sadati Khadar, Ali Mohamadi Sani

Department of Food Science and Technology, Quchan Branch, Islamic Azad University, Quchan, Iran

Key words: Bottled water, membrane filter culture (MFC), polymerase chain reaction (PCR), P. aeruginosa.

Abstract

waterbottlesOver the recent years the consumption of bottled water has increased tremendously, and this trend is expected to continue, demanding the continuous surveillance of public health service. The propose of this study was to determine the microbial quality to Pseudomonas aeruginosa by membrane filter (MFC), enrichment culture and polymerase chain reaction (PCR) method of commonly available brands of bottled water in Iran. In this cross sectional study, 52 sample from 17 bottled water of available brands of domestically produced were examined by membrane filter (MFC) culture and polymerase chain reaction (PCR) methods for detection of P. aeruginosa. Results showed that none of the 52 samples tested were positive for total coli forms and E. coli. The MFC method showed that 14 (26.9%) of all 52 water samples were positive, the culture method showed that 8 (15.3%) were positive and by PCR as gold standard 5 (10.4%) of all samples were positive for P. aeruginosa. This study showed that PCR method can be an extremely high specificity and sensitivity, safety and can be served as a gold standard for monitoring of drinking waters.

Get the original articles: http://www.innspub.net/volume-6-number-4-february-2015-ijb/

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