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Genetic structure in anzali wetland’s pike (Esox lucius) using microsatellite molecular method

Mohammad Hadi Samiei, Mehrnoush Norouzi, Amin Ravaei, Mohammad Behrooz, Ali Nazemi

Department of Marine Biology and Fishery, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran

Department of Biology, Faculty of Sciences, Kharazmi University, Tehran, Iran

Department of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran

Key words: Genetic structure, Esox lucius, Anzali Wetland, Microsatellite.


northern-pike-80658In this research, Pike (Esox lusius) one of the most valuable commercial marine species has been evaluated for genetic structure in Anzali wetland using microsatellite markers. 60 specimens of adult pikes were sampled from two spawning seasons, winter and spring in Anzali wetland. Five pairs of microsatellites, tested on the genomic deoxyribonucleic acid (DNA). All loci of microsatellite produced polymorphic bands as polymorphic loci were used to analyze the genetic variation of the pike. Analyses revealed that average of alleles per locus were 10.8 (range 9 to 13 alleles). All sampled seasons contained private alleles. The average observed and expected heterozygosity was 0.914 and 0.885, respectively. The inbreeding coefficient values of five microsatellite loci were negative. With the exception of a locus in spring, all loci significantly deviated from H-W equilibrium (P<0.01). Based on AMOVA, RST and FST values were significant between seasons (P<0.01). The genetic distance between populations was 0.442, which indicates that the genetic difference among the studied populations is significant. These results support the existence of different genetic populations in spawning seasons in this area.


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