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Genotyping of Salmonella enterica subsp. enterica serovar Entritidis, isolated from poultry, cattle and human in Iran by ERIC-PCR

Abbas Ali-Shiroodi, Mahmood Jamshidian, Taghi Zahraei Salehi, Gholam Reza Nikbakht Boroujeni, Kumarss Amini

Department of Pathobiology, College of Veterinary Medicine, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran,Iran

Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran

Key words: Salmonella Entritidis, ERIC-PCR, Human, Poultry, Cattle, molecular profile.

Abstract

Cattle-in-FranceSalmonellosis is a serious disease in human and animals. One of the most important serovars (among more than 2610 known serovars of Salmonella) is Salmonella enterica serovar Entritidis which is a common foodborne pathogen worldwide. Various molecular subtyping techniques have been applied for identification of Salmonella. Enterobacterial repetitive intergenic consensus (ERIC) PCR techniques [collections of ERIC1R–ERIC2 primers] were examined for the discrimination of Salmonella enterica seovar Entritidis isolates at the serotype level. Sixty four S.Entritidis isolates from both human and (non-human) sources (cattle and poultry) in Iran, which previously were described by common culture, biochemical and serological procedures, were chosen. Enterobacterial repetitive intergenic consensus (ERIC) were selected for rep-PCR to generate DNA fingerprints for the S.Entritidis isolates. These banding patterns provided DNA fingerprints of different isolates. A dendogram was made by using NTSYS software, Version 2.0 and represented that most human isolates were separately clustered from the non-human isolates. This experiment also revealed that PCR fingerprinting with the ERIC primers is suitable for typing of the S.Entritidis isolates from different origin and for epidemiological trace back and taxonomy.

Get the original articles: http://www.innspub.net/volume-6-number-3-february-2015-ijb/

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