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Haplotype analysis of molecular markers linked to QTLs controlling Iron content in rice grains

Shahrbanu Abutalebi, Mohammad-Hossein Fotokian, Mehrshad Zeinalabedini

Department of Agronomy and Plant breeding, Faculty of Agriculture, Shahed University, Tehran, Iran

Agriculture Biotechnology Research Institute of Iran, Karaj, Iran

Key words: Allelic pattern, Iron, Rice grain, SSR marker.

Abstract

Rice-grainIron deficiency affects 2 billion people currently and the number is increasing. Biofortification of rice is one of the best approaches for solution this problem. Molecular marker techniques can greatly improve the efficacy of breeding programs to improve grain iron content and bioavailability in major staple crops such as rice. In the current study, the haplotype variation of three loci controlling iron content was evaluated using 50 genotypes and 14 associated microsatellite markers. The grain iron content analysis of a collection of 50 rice genotypes showed large variation from 9.06 (Sepidrud cultivar) to 50.55 (Mehr cultivar) mg.kg-1 indicating the existence of genetic potential to increase the content of this micronutrient in rice grain. Based on the results of genotyping RM276 indicated highest polymorphism information content (0.83). The haplotype diversity analysis showed, allelic pattern of 132-176-200 bp in the Norin22 and Shahak cultivars on chromosome 12 had the most similarity with haplotype of reference cultivar Mehr. This allele combination can be informative markers for improvement of iron content in rice grains through marker-assisted selection programs. Furthermore, the presence of allele combinations, different from reference haplotype in Dadras and Farideh cultivars (with high iron contents) indicated the presence of novel source of QTLs controlling grain iron content in Iranian rice cultivars.

Get the original articles: http://www.innspub.net/volume-6-number-5-may-2015-jbes/

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