Rhimi Awatef, Hannachi Hédia, Hjaoujia Sonia, Yousfi Haifa, Boussaid Mohamed
Laboratory of Plant Biotechnology, National Gene Bank of Tunisia (NGBT). Tunis Cedex, Tunisia
Department of Biology, National Institute of Agronomy of Tunis (INAT), Tunis- Mahrajène, Tunisia
Department of Biology, National Institute of Applied Sciences and Technologies (INSAT). Tunis Cedex, Tunisia
Key words: Micropropagation. In vitro pretreatments, Caper populations.
Capparis spinosa L. (Capparaceae) is a perennial tropical and subtropical shrub plant known worldwide. Despite the increasing demand and economic importance of capers, little information is available regarding the propagation of this shrub. In fact, it is usually propagated by seed, but its percentage of germination is very low. In this report, in vitro seed germination and seedling development of four caper populations were studied and several treatments were evaluated to determine the ability of several factors to increase the percentage of germination. The seeds were treated with sulfuric acid (H2SO4) for 30 min. These seeds were left out in two different germination media (MS and H2O), for 30 days by soaking them in 1000 or 2000 mg.l-1 of GA3 each for 6, 12, 24 or 48 h. High variability was observed among the germination percentages of the different treatments varying from 4.16 to 75%. The variability was due to the Gibberellic acid level (GA3), to the genotype and to the media culture. The highest germination rate of 75% was obtained from the Nahli site seeds treated with 2000 mg.l-1 GA3 for 48 hours. For all populations, regenerated plants were transplanted in pots on a sterile substrate. The rate of survival plants after acclimatization was 100%.
Get the original articles in Source: In vitro germination and seedling development of tunisian caper (Capparis spinosa L.)
Published By: International Network for Natural Sciences