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In vitro screening of pea genotypes tolerant to iron deficiency based on physiological traits

Imran Ahmed, Ahmad Humayan Kabir, Mohammad Farhadur Rahman, Mohammad Firoz Alam

Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Key words: In vitro culture, Fe deficiency tolerance, peas, chlorophyll concentration, screening.


tissue-culture-1Screening of genotypes tolerant to Fe deficiency was performed in a number of Australian (Santi, Parfield, BC11, BC191, BC 17 and BC14) and Bangladeshi (BARI-1 and IPSA-2) genotypes based on different physiological parameters. Fe deficiency caused severe decrease in chlorophyll a and b concentrations in Parafield, BC17 and IPSA-2 grown on MS (Murashige and Skoog) media on in vitro conditions. In contrast, chlorophyll a and b concentrations were not significantly decreased in Santi, BC11, BC91, BC14 and BARI-1. Furthermore, number of leaves, shoot height and weight were significantly reduced in Parafield, BC17, BD14 and IPSA-2; whereas Santi, BC11, BC91 and BARI-1 did not show prominent decrease in the aforesaid growth parameters due to Fe deficiency. Again, Parafield, BC17 and IPSA-2 showed significant decrease in root length and root biomass under Fe deficiency. In contrast, these parameters were unchangeable in Santi, BC11, BC91, BC14 and BARI-1 in Fe shortage compared to controls. Based on these findings, tolerance to Fe deficiency in these pea genotypes can be categorized as: tolerant (Santi, BC11, BC91, BARI-1), intermediate (BC14) and sensitive (Parafield, BC17, IPSA- 2). This study demonstrates the effectiveness of in vitro culture as an efficient method to screen Fe-efficient crop plants. Moreover, the ranking can be applied in plant breeding program and may have great advantage over conventional methods.


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