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Increasing of Chavicol o-methyl transferase gene expression (CVOMT) and methyl Chavicol value of basil (Ocimum basilicum) by salicylic acid

Hemadollah Zarei, Barat Ali Fakheri, Sedigheh Esmaeilzadeh Bahabadi, Mahmood Solouki

Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, IR Iran

Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, IR Iran

Department of Biology, University of Zabol, Zabol, IR Iran

Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, IR Iran

Key words: basil, essential oil, gene expression, CVOMT, salicylic acid.

Abstract

tomato_basilBasil (Ocimum basilicum), is a medicinal plant mint family (Lamiaceae) which contains cyclic compounds and essential oil, and it has antibacterial and antioxidant properties. From Long ago, Basil has been used for treatment of headache, cough, diarrhea and fever, inflammation of the throat and stomach pain in Iran. Basil essential oil is mainly containing phenylpropanoids compounds, the biosynthesis path of these compounds are of course passed from the pathway of shikimate and the most important compounds of them, that can be cited, are chavicol and methyl chavicol. The role of salicylic acid is known as a bio- stimulant to improve the biosynthesis of secondary metabolites in many plants. In this study, the effect of salicylic acid on gene expression CVOMT and the amount of methyl chavicol was studied. Therefore, basil was treated with 2 mM solution of salicylic acid before flowering stage, and respectively the samples were harvested 1, 2, 3 and 5 days after treatment. Gene expression CVOMT which was under influence of salicylic acid, compared with controls, showed the highest activity of gene expression CVOMT. So, on the third day after treatment was reached to highest level and on the fifth day after treatment was reduced. Essential oil analysis also showed that the methyl chavicol influenced by salicylic acid, in comparing with controls, like gene expression (CVOMT) was increased. In General, the changes in gene expression CVOMT and the amount of methyl chavicol that harvested at different stages were Conformity.

Source: http://www.innspub.net/wp-content/uploads/2015/03/JBES-Vol6No3-p46-53.pdf

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