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Isolation, characterization and antibiotic susceptibility of Escherichia coli and Salmonella spp. isolated from local beverages (bissap, gnamakoudji) sold in Ouagadougou, Burkina Faso

Gertrude Bsadjo Tchamba, Hadiza Ibrahim Bawa, Ariane Nzouankeu, Touwendsida Serge Bagré, Alfred S. Traoré, Nicolas Barro

Laboratoire de Biologie Moléculaire, d’Epidémiologie et de Surveillance des Bactéries et Virus

Transmissibles par les Aliments (LaBESTA), Centre de Recherches en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Ecole Doctorale Sciences et Technologies, 03 BP 7021 Ouagadougou 03, Université de Ouagadougou, Burkina Faso

Centre Pasteur du Cameroun, BP 1274 Yaoundé, Cameroun

Key words: Local beverages, Escherichia coli, EPEC, Salmonella spp., antibiotic resistance.


Concerns-raised-by-salmonella-enterica-antibiotic-resistance_medium_vgaBissap» and «gnamakoudjii» are two local beverages highly produced and consumed in Burkina Faso, but potential hazard related to unhygienic contamination can cause a major risk to the health of consumers. This study was performed to assess the prevalence and antibiotic resistance of Salmonella spp. and Escherichia coli in 150 samples of «gnamakoudjii» and 150 samples of «bissap» sold in twenty four open markets in Ouagadougou city. Samples were aseptically collected and analyzed using standard microbiology methods within an hour of procurement. Slide agglutination with 12 enteropathogenic E. coli (EPEC) antisera was used for the detection of EPEC. Nineteen (12.70%) and thirty one (20.70%) strains of E. coli were respectively isolated in «bissap» and «gnamakoudjii». Among E. coli isolated, 7 (14%) E. coli isolates were serotyped as EPEC belonging to 4 serotypes (E. coli O86; E. coli O119; E. coli O126 and E. coli O128). No Salmonella were detected in «gnamakoudjii» while 2 (1.33%) strains of Salmonella were isolated in «bissap». Antibiotic resistance results indicated that E. coli isolates were resistant to amoxicillin-clavulanate, chloramphenicol, cefamandole, aztreonam, cephalothin, nalidixic acid, ticarcillin and tetracycline. Training/retraining on hygiene and sanitation is recommended to avoid contamination of local beverages.


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