L. Ghaffarzadeh-Namazi, N. Babaeian, A. Ghamari-zare, GH. Nematzadeh
Department of Plant Breeding at Sari Agricultural Sciences and Natural Resources University, Sari, Iran
Research Institute of Forests and Rangelands, Tehran, Iran
Key words: Satureja, cryopreservation, germplasm, glycerol, vitrification.
Many species of the genus Satureja are reported to have aromatic and medicinal properties. Seed storage techniques at very low temperature (-196 °C) using liquid nitrogen is one way of maintaining germplasm with much less cost and without loss of viability on storage. In order to preserve Satureja bachtiarica seeds under – 196 ºC condition, three protocols, Plant vitrification solution (PVS2), Desiccation and Glycerol were studied. The treated seeds were transferred into Liquid Nitrogen (LN) and stored for 7, 30 and 90 days respectively. In this study the effect of cryopreservation on germination and growth indices (germination percentage, germination rate) of plant species (S. bachtiarica) in storage conditions of cryopreservation (-196ºC) were evaluated for 7, 30 and 90 days. This study showed that liquid nitrogen did not affect growth indices (germination percentage, germination rate) from cryopreserved seeds when compared with control seeds. So the seed of this endangered species can be preserved for a long period. According to the results there were no significant differences among 7, 30 and 90 days liquid nitrogen storage treatments in trait germination percentage and rate. This study revealed that cryopreservation did not have significant effects on seed germination percentage and germination rate of this species. Therefore cryopreservation is a suitable procedure for the long-term preservation of S. bachtiarica.
Source/In English: http://www.innspub.net/wp-content/uploads/2015/01/IJB-V6No2-p24-29.pdf