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Lactococcus lactis as an oral vector for cloning of heat shock protein A from Helicobacter pylori

Samaneh Dolatabadi, Tahereh Falsafi, Mahmoud Mahmoudi

Department of Microbiology, Faculty of Biology, Alzahra University, Tehran, Iran

Immunology Research Center, BuAli Research Institute, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Key words: Helicobacter pylori, Heat shock protein A, Probiotic, Lactococcus lactis.


h-pylori-and-ulcersLactococcus lactis, a safe probiotic bacterium would be one of the potential candidates for cloning. The aim of present study was to clone H. pylori heat shock protein A (hspA) in Lactococcus lactis for application in immunological studies. hspA gene sequence of H. pylori 26695 containing His-tag-Opt was constructed and was inserted into E. coli-L. lactis shuttle vector pNZ8148, transformed into E. coli MC1061, and its insertion was confirmed by PCR. The purified recombinant plasmid was introduced into L. lactis NZ9000 by electrotransformation. The presence of hspA gene in pNZ8148 shuttle vector, as well as in L. lactis NZ9000 transformants was confirmed by enzymatic digestion, PCR and sequencing of cloning products. Due to the safety and importance of probiotic bacteria, the recombinant L. lactis NZ9000 expressing H. pylori hspA protein would be a promising oral vaccine candidate against H. pylori.

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