Fida Muhammad Abbasi, Kiran Zubair, Muniba Fida Abbasi, Muhammad Sajid, Ashiq Rabbani
Department of Genetics, Hazara University Mansehra, Pakistan
Plant Genetic Resources Institute, NARC Islamabad, Pakistan
Key words: Interleukin-10 gene, Type 1 diabetes, T1DM.
Type 1 diabetes mellitus (T1DM) caused by the destruction of insulin producing beta cells of pancreas that are responsible for the autoimmune system of the individuals. Blood and urine glucose increases due to the lack of insulin in the body. The objective of the present study was to evaluate the role of IL-10 gene polymorphisms as susceptibility markers for Pakistani patients. IL-10 promoter polymorphisms (positions-592A/C) were analyzed in 183 individuals of Abbottabd. Ninety three known Pakistani patients with type-1 diabetes including 49 men and 44 women were recruited; having age ranged from 1.0 –70.0 years. Ninety one healthy individuals with no family history of diabetes were included as control. RFLP and PCR were used to detect allelic polymorphism of the IL-10 gene promoter region at position -592. Results indicated that the genotype frequency of the allel (- 592A) was associated with type-1 diabetes. The genotype frequency of -592A allele was higher in adult patients than in old age patients. Our investigation suggests that IL-10 gene polymorphisms could be involved in the development of type-1 diabetes in the Pakistani population.