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New primer generated bacterial mapping and biofertilizing potentiality assessment of Pseudomonas sp. isolated from cowdung

Gopinath Rana, Arindam Adhikari, Tanusri Mandal

Bioconversion Laboratory, Department of Biotechnology, Vidyasagar University, Rangamati, Paschim Medinipur, West Bengal, India

Key words: Cowdung, plant growth promoting fluorescent Pseudomonads, MEGA6; species specific primer design, Fast PCR.

Abstract

Cow-dung-patties-popular-in-India-go-on-sale-on-Amazon-and-eBayModern biofertilizer trend is to use of different plant growth-promoting microorganisms (PGPM), isolated from several sources, including rhizosphere. Here we evaluate the plant growth promoting ability of some fluorescent Pseudomonads, isolated from cow dung. Based on different biochemical tests, we select 7 isolates to observe their effect on Cicer arietinum (Bengal gram) seed germination and sprout growth promotion. A new Pseudomonas sp Strain TMGR (NCBI Accession Number JX094352) showed best effect with Vigor-index value 4470. 16s rRNA gene sequencing revealed that all isolates have 97-99% similarities with Pseudomonas aeruginosa. On the basis of Molecular Evolutionary Genetics Analysis version 6.0 (MEGA6), analysis of 16S rRNA sequences of selected seven isolates with other native Pseudomonas aeruginosa strains were available in GeneBank, a phylogenetic tree was constructed to study their relative position. Adopting BioEdit and Fast PCR software programs, we designed Pseudomonas aeruginosa specific two primers, 103F and 455R, and their validity was rechecked in wet laboratory. These primers may help in isolation and rapid identification of plant growth promoting Pseudomonas aeruginosa strains from different sources in future.

Get the original articles: http://www.innspub.net/volume-5-number-5-november-2014-ijaar/

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