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Phosphate removing using bacteria isolated from activated sludge for wastewater remediation

Oumaima Naili, Messaoud Benounis, Leyla Benammar

Laboratory of Sensors, Instrumentations and Process (LCIP), Department of Chemical Engineering, Institute of Science and Technology, University of Khenchela, Algeria

Laboratory of Applied Microbiology, University of Setif, Algeria. 3Laboratory ESPA, University of Batna, Algeria

Key words: Batch culture, Biological treatment, Activated sludge, Phosphate reduction, Waste water.


waste-waterThe objective of the current study is to isolate phosphate removing microbes from activated sludge for wastewater remediation in the area of Khenchela (Eastern Algeria) . Phosphate rate was determined using colorimetric method and batch tests were developed to evaluate the biomass composition of the sludge. Four efficient pure strains isolated from activated sludge samples and identified as Acinetobacter junii, Pseudomonas aeruginosa, Moraxella lacunata, Alcaligenes denitrificans were tested for their capacities of phosphate reduction. The best rate of phosphate removal by pure cultures is observed with Acinetobacterjunii, 76%, followed by Alcaligenes denitrificans, 70.42%, Pseudomonas aeruginosa, 61.78%, and Moraxella lacunata, 50.6%. Our results show that applying of mixed bacterial culture containing mostly isolated strains for bioremediation purpose can be used successfully for the elimination of phosphate from activated sludge of wastewater treatment plants.

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