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Screening identification and characterization of lipase producing soil bacteria from upper dir and Mardan Khyber Pakhtunkhwa, Pakistan

Nadeem Ullah, Muhammad Daud, Hidayatullah, Muhammad Shabir, Ayşegül Taylan Özkan, Sami ullah, Muhammad Qasim.

Faculty of Medicine, Department of Microbiology, Near East University, Turkish Republic of Northern Cyprus

 Department of Microbiology, Kohat University of Science and Technology Kohat, Khyber Pakhtunkhwa, Pakistan

 Department of Microbiology, Hazara University Manshera, Dhodial, Khyber Pakhtunkhwa, Pakistan

Department of Biosciences, Comsat Institute of Information Technology, Islamabad, Pakistan

 Center of Biotechnology and Microbiology, University of Peshawar KhyberPakhtoonkhwa, Pakistan

Key words: Lipase, dir, bacteria, gram negative.


Dir upper1Microbial lipases are one of the most important extracellular metabolites which have been the main focus of scientific research due to its huge biotechnological usage over the years. Although there are many sources of lipases but microbes are the best producers. In this regards, the present work was conducted to isolate and screen out lipolytic bacteria from soil of Upper Dir and Mardan areas. Later on the lipolytic activity was observed through optimization of cultural physico-chemical parameters. Screening of lipolytic bacteria was achieved by inoculation of sample on selective medium that is tributyrine agar. Out of thirty (30) soil samples, Twenty one (21) were lipase producers. Fifteen (15) were gram positive and six (6) were gram negative. Bacillus spp were further explored for growth and lipase production optimization. Bacillus spp produce optimum growth at 37oC and pH 7. Furthermore, the optimum enzyme production for Bacillus spp was observed at pH 7 temperature 37°C and at 48hours time duration.

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