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Screening of MDR-bacteria from fecal specimens of AAD patient and inhibit them using fruits extracts of Moringa oleifera Lam

Md. Sarwar Parvez, Most. Ferdousi Begum, Farzana Ashrafi Neela, Mohammad Firoz Alam

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Antibiotic-associated diarrhea (AAD), Antibiotic susceptibility, Multidrug-resistant bacteria, Moringa oleifera Lam., Antibacterial activities.


DrumstickFlowerThe present research was undertaken to screen of multidrug resistant bacteria (MDRB) from fecal specimens of Antibiotic-associated diarrhea patient and to evaluate the potentiality of M. oleifera fruits extracts on those bacteria with the view to provide scientific evidence for its application in health remedy. MDRB were determined by antibiotic (tetracycline, azithromycin, ciprofloxacin & erythromycin) susceptibility test, using disc-agar diffusion method. Biochemical tests of the MDRB were done according to Bergey’s Manual of Determinative Bacteriology for identification of the species. Minimum inhibitory concentration (MIC) as well as minimum bactericidal concentration (MBC) of the extracts was also measured. Among the 13 isolated bacteria only 5 were found resistant to multiple antibiotics. The fruit extract showed a broad-spectrum antibacterial activity against isolated four species (Escherichia coli, Shigella sp., Salmonella sp. & Klebsiella sp.) of MDRB. Among used two solvents, methanol possessed better antibacterial activity than ethanol. The highest zone of inhibition (19.86mm) was found in methanol at the concentration of 500mg/ml for E. coli. The MIC and MBC values were determined as 300mg/ml and 400mg/ml respectively. The consequences of this investigation suggest that the extracts of M. oleifera Lam. can be used to discover antibacterial agent for developing new pharmaceuticals to control different severe illness caused by MRD bacteria.

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