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Study of pectinolytic activity of Fusarium oxysporum f.sp albedinis agent responsible for bayoud in Algeria

Karkachi Noureddine, Gharbi, Samia, Kihal Mebrouk, Henni Jamal Eddine

Department of Biology , University of Oran, Algeria

Department of Biotechnology University of Sciences Technologies Oran (USTO), Algeria

Key words: Fusarium oxysporum f.sp albedinis, pathogenicity, pectinmethylesterase (PME), polygalacturonase (PG), citrus pectin.


hanging-orangesThe present study is devoted to the morphological characterization, pathogenicity of Fusarium oxysporum f.sp albedinis, which showed morphological variability of isolates. The study of pectin methyl esterase and polygalacturonase activities by reducing sugars dosages of 10 isolates, showed that the PME and PG activities are present in the culture filtrates of isolates and the estimated amount is much higher in the presence of citrus pectin and glucose. We noted that this activity is variable among isolates, high activity is recorded (1.59 103 U/mole) in presence of glucose but it was greater than 4 times in the presence of citrus pectin, The PG activity is recorded (1.39 103 and 0.44 103μegr) in the presence of 1% citrus pectin and glucose respectively.

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