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Studying the effects of erythropoietin in the prevention of liver inflammation and subsequent cell death induced by ischemia-reperfusion in rats

Seyyed Saied Disnad, Yousef Doustar

Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Key words: Liver Ischemic reperfusion, erythropoietin, cell death.


ischemic_liver_transplant_12_5EPO is a cytokine that was originally identified as the major regulator of erythroid precursor cells. However EPO attenuates cell damage and inflammatory reactions. In the present study, we examined the effect of EPO on liver ischemic reperfusion injury and also on the occurrence of cell death and inflammatory response in an in vivo rat model of liver I/R injury. Studies were performed on male wistar rats weighing 200 to 250 g (n = 8). Rats received a standard diet and water ad libitum and were housed in a 12-h light/dark cycle. The animals were randomly allocated into two groups: (1) I/R-saline group, in which rats were subjected to renal ischemia for 30 min (n = 8); (2) I/R-EPO group, in which rats were administered EPO (500 U/kg, i.p.) 60 min prior to I/R (n = 8)in which rats were subjected to identical surgical procedure without occlusion of both portal and liver artery pedicles and maintained under anesthesia for the duration of the experiment (n = 8). in group 2 erythropoietin caused significant decrease cell death and inflammatory reactions in comparison with control group (p<0.001). Results indicated that erythropoietin inhibits cell death and inflammatory reactions in the liver rats.

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