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The effect of 2,4-D on callus induction using leaf lobe of sweet potato as a source of explant

F.Addae-Frimpomaah, E. Arkorful, T.K. Tengey

Savanna Agricultural Research Institute, Nyankpala-Tamale, Ghana

Graduate School of Nuclear and Allied Sciences, Department of Nuclear Agriculture and Radiation Processing, University of Ghana, Atomic-Accra, Ghana

Key words: Callusogenesis, 2, 4-dichlorophenoxyacetic acid, CLC/ Ipomoea medium, leaf lobes.

Abstract

sweetpotatoThe effect of 2, 4-dichlorophenoxyacetic acid (2,4-D) on young leaf lobes of three sweet potato accessions UE007, UK-BNARI and SA-BNARI for callus induction was investigated. Callusogenesis was achieved when leaf lobe explants from four weeks old healthy growing plantlets of three sweet potato accessions SA-BNARI, UKBNARI and UE007 were cultured on CLC/ Ipomoea medium supplemented with 1.0 – 4.0 mg/l 2,4-D with 4.0 mg/l 2,4-D being the optimal concentration. However, the calli were non-embryogenic and therefore could not produce embryos when transferred to 0.1 mg/l BAP amended medium but rather produced either single or multiple shoots. The highest percentage shoot (83.3 %) was obtained from 4.0 mg/l 2,4-D-derived callus. These results indicate that indirect shoot development via a callus phase is a feasible option for sweet potato propagation using in vitro techniques.

Get the original articles: http://www.innspub.net/volume-5-number-1-july-2014-ijaar/

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