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The effect of activated charcoal and multi-walled carbon nanotubes on the growth, rosmarinic and caffeic acid content, total phenol, total flavonoid and antioxidant activity of Satureja rechingeri calluses

Hassan Esmaeili, Javad Hadian, Mohammad Hossein Mirjalili, Hassan Rezadoost

Department of Agriculture, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, G.C., Tehran, Iran

Department of Phytochemistry, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, G.C., Tehran, Iran

Key words: Satureja rechingeri, Rosmarinic acid, Multi-walled carbon nanotubes, Activated charcoal, Antioxidant activity.

Abstract

nepeta_sphaciotica-9largeSatureja rechingeri belongs to the Lamiaceae family and is native to Iran. The plant produces high amounts of phenolic compounds, especially rosmarinic acid. The effects of multi-walled carbon nanotubes with a carboxyl functional group (MWCNT-COOH) and activated charcoal (AC) on the growth, rosmarinic and caffeic acid content, total phenol, total flavonoid and antioxidant activity of S. rechingeri calluses were evaluated. MWCNT-COOH and AC were able to increase the amount of fresh and dry weight compared to control in a wide range (25-500 μg/ml). The maximum amount of rosmarinic acid was observed in concentrations of 100 μg/ml MWCNT-COOH and 250 μg/ml AC. Positive correlations between antioxidant activity and total phenol content and rosmarinic acid content were observed. These data underline the role of rosmarinic acid as a potent antioxidant in plants.

Get the original articles: http://www.innspub.net/volume-6-number-5-may-2015-jbes/

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